Products
MagDot 655 nm CD4 (RPAT4) – 20 Tests
$420.00
The MagDot is a dual-purpose reagent, it combines quantum dots and superparamagnetic iron oxide particles within the same nanoparticle. Cells labeled with MagDots targeting specific antigens will be both fluorescent and magnetic. Therefore, after labeling cells of interest, one can look at the feed to determine the percent positive fluorescently via flow cytometry, and then place the labeled cells in a magnetic separator and analyze both the magnetic (for the enrichment) and the nonmagnetic (for the depletion) of targeted cells without the need for further fluorescent labeling.
Mouse anti human CD4 antibody, clone RPA-T4 conjugated to this MagDot recognizes human CD4, a 55 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale.
Product Information
|
Number of tests |
20 tests |
|
Dilution* |
15-20 µL/million cells |
|
Antibody Concentration |
0.08 mg/mL |
|
Host/Isotype |
Mouse/IgG1 |
|
Particle Concentration |
1011 particles/mL |
|
Iron Concentration |
0.5 mg/mL |
|
Class |
Monoclonal |
|
Type |
Antibody |
|
Clone |
RPAT4 |
|
Immunogen |
Human CD4 |
|
Conjugate |
655 nm MagDot |
|
Form |
Liquid |
|
Purification |
Purified |
|
Storage Buffer |
Borate, pH 7.4 with 0.5% BSA, 0.1% sodium azide |
|
Storage Conditions |
4˚C do not freeze |
|
Reactivity |
Human |
|
Tested Application |
Flow Cytometry and Magnetic Separation |
|
Catalog Number |
MagD-CS-655-H4 |
*Suggested working dilution is given as a guide only. It is recommended the user titrate the product for use in their own experiment using appropriate negative and positive controls.
*Time for separation for the labelled cells will depend on magnetic field strength, gradient used and no of particle bound/ cell.
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